Hotspot

psbF-psbE

LSC · 71131-71139 · 9 bp

Back to Hydnocarpus

Pi 0.111

MarkerSeek score 80.20

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	GGTAGGTACAGCTAGTCCGT	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	136 bp	1.0	80.2
psbF-psbE_p2	GGTAGGTACAGCTAGTCCGT	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	135 bp	1.0	80.2
psbF-psbE_p3	ACGGTAGGTACAGCTAGTCC	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	138 bp	1.0	80.1
psbF-psbE_p4	ACGGTAGGTACAGCTAGTCC	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	137 bp	1.0	80.1
psbF-psbE_p5	AGGTACAGCTAGTCCGTGAA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	57.8	58.0	132 bp	1.0	78.9