Hotspot

trnS-UGA-lhbA

LSC · 41385-41764 · 380 bp

Back to Hydnocarpus

Pi 0.0316

MarkerSeek score 70.70

Primer available yes

Label trnS-UGA-lhbA

Feature Metadata

label_name: trnS-UGA-lhbA
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 12
pi: 0.0316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-lhbA_p1	CAAGACCGGAGCTATCAACC	TGCTAGACCAACCATCAGGA	55.0	50.0	58.1	58.0	520 bp	1.0	81.0
trnS-UGA-lhbA_p2	CTATCAACCACTCGGCCATC	TGCTAGACCAACCATCAGGA	55.0	50.0	58.1	58.0	509 bp	1.0	80.5
trnS-UGA-lhbA_p3	GAACCCTCGATAGTTCGCAA	TGCTAGACCAACCATCAGGA	50.0	50.0	57.7	58.0	557 bp	1.0	79.0
trnS-UGA-lhbA_p4	ACCCTCGATAGTTCGCAAAG	TGCTAGACCAACCATCAGGA	50.0	50.0	57.7	58.0	555 bp	1.0	79.0
trnS-UGA-lhbA_p5	GATTCGCCGAGAAAAGGAGA	TGCTAGACCAACCATCAGGA	50.0	50.0	57.7	58.0	588 bp	1.0	78.9