Hotspot

rpoB-trnC-GCA

LSC · 30343-31605 · 1263 bp

Back to Hydnocarpus

Pi 0.019

MarkerSeek score 62.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1263
variable_sites: 24
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAAGCTCTTCTGCTAAGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1431 bp	1.0	79.7
rpoB-trnC-GCA_p2	AAAGCTCTTCTGCTAAGCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1413 bp	1.0	77.5
rpoB-trnC-GCA_p3	CCTCATTTCTATCCCCGAGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.5	58.1	1347 bp	1.0	77.1
rpoB-trnC-GCA_p4	AAGCTCTTCTGCTAAGCCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.7	58.1	1430 bp	1.0	75.0
rpoB-trnC-GCA_p5	CCTCATTTCTATCCCCGAGC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.5	58.4	1329 bp	1.0	74.9