Hotspot

atpH-atpI

LSC · 16806-17985 · 1180 bp

Back to Hydnocarpus

Pi 0.00932

MarkerSeek score 64.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1180
variable_sites: 11
pi: 0.00932
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	CCATGGAAGGGCATCATTGA	45.0	50.0	58.1	57.9	1230 bp	1.0	79.3
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	TCCATGGAAGGGCATCATTG	45.0	50.0	58.1	57.9	1231 bp	1.0	79.3
atpH-atpI_p3	ACGGAAGCAGCAGAAATCAA	CCATGGAAGGGCATCATTGA	45.0	50.0	58.1	57.9	1229 bp	1.0	79.3
atpH-atpI_p4	ACGGAAGCAGCAGAAATCAA	TCCATGGAAGGGCATCATTG	45.0	50.0	58.1	57.9	1230 bp	1.0	79.3
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	CCATGGAAGGGCATCATTGA	50.0	50.0	57.9	57.9	1270 bp	1.0	79.1