Hotspot

psbI-trnS-GCU

LSC · 9953-9991 · 39 bp

Back to Hydnocarpus

Pi 0.0513

MarkerSeek score 69.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 39
variable_sites: 2
pi: 0.0513
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	TTATTCGTACCGAGGGTTCG	50.0	50.0	58.3	57.2	110 bp	1.0	72.5
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	TTCGAATCCCTCTCTTTCCG	50.0	50.0	58.3	57.0	94 bp	1.0	71.9
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	GGGTTCGAATCCCTCTCTTT	50.0	50.0	58.3	56.9	97 bp	1.0	70.9
psbI-trnS-GCU_p4	ATGATCCAGGGCGTAATCCT	GGGTTCGAATCCCTCTCTTTC	50.0	52.4	58.3	58.1	97 bp	1.0	70.8
psbI-trnS-GCU_p5	ATGATCCAGGGCGTAATCCT	GGTTCGAATCCCTCTCTTTCC	50.0	52.4	58.3	58.1	96 bp	1.0	70.8