Hotspot

rps16

Genome · 70989-71958 · 970 bp

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Pi 0.0151

MarkerSeek score 54.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 970
variable_sites: 28
pi: 0.0151
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGTCGCAGTTTCACAAACCT	CCGGGCATGGAAGATCATTT	45.0	50.0	58.0	58.3	2206-2209 bp	1.0	79.6
rps16_p2	AGTCGCAGTTTCACAAACCT	ACGCCTGAATATTCCCTGTG	45.0	50.0	58.0	57.7	2336-2339 bp	1.0	79.4
rps16_p3	AGTCGCAGTTTCACAAACCT	CGCCTGAATATTCCCTGTGG	45.0	55.0	58.0	58.4	2335-2338 bp	1.0	78.8
rps16_p4	TCCCTTCAGGATTAGTCGCA	CCGGGCATGGAAGATCATTT	50.0	50.0	58.1	58.3	2219-2222 bp	1.0	78.8
rps16_p5	TCCCTTCAGGATTAGTCGCA	ACGCCTGAATATTCCCTGTG	50.0	50.0	58.1	57.7	2349-2352 bp	1.0	78.5