Hotspot

rpoC2

Genome · 85088-90304 · 5217 bp

Back to Huperzia

Pi 0.0118

MarkerSeek score 50.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: Genome
length_bp: 5217
variable_sites: 115
pi: 0.0118
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCTTCGGTTGCTTCACCAA	CGAGGTCGAATCGATTGGTT	45.0	50.0	58.0	58.1	450-552 bp	1.0	86.4
rpoC2_p2	TTTCTTCGGTTGCTTCACCA	CGAGGTCGAATCGATTGGTT	45.0	50.0	58.0	58.1	451-553 bp	1.0	86.4
rpoC2_p3	TTCTTCGGTTGCTTCACCAA	GTGAATTCTGCTTCGGGGAA	45.0	50.0	58.0	58.2	588-690 bp	1.0	84.9
rpoC2_p4	TTCTTCGGTTGCTTCACCAA	AGTCTGGAGCAGAGTCAAGT	45.0	50.0	58.0	58.0	780 bp	0.25	49.6
rpoC2_p5	TTTCTTCGGTTGCTTCACCA	AGTCTGGAGCAGAGTCAAGT	45.0	50.0	58.0	58.0	781 bp	0.25	49.6