Hotspot

rrn23-trnA-UGC

Genome · 146314-147274 · 961 bp

Back to Huperzia

Pi 0.0245

MarkerSeek score 81.00

Primer available yes

Label rrn23-trnA-UGC

Feature Metadata

label_name: rrn23-trnA-UGC
feature_type: igs
region: Genome
length_bp: 961
variable_sites: 42
pi: 0.0245
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-trnA-UGC_p1	GTCTCTGGGTGCCTAGGTAT	TCCCGGCCTTCTTGGTATAA	55.0	50.0	57.9	58.1	1100-1271 bp	0.5	63.9
rrn23-trnA-UGC_p2	GTCTCTGGGTGCCTAGGTAT	TTCCCGGCCTTCTTGGTATA	55.0	50.0	57.9	58.1	1101-1272 bp	0.5	63.9
rrn23-trnA-UGC_p3	GAATCTCCGGATCCATGCTC	TCCCGGCCTTCTTGGTATAA	55.0	50.0	57.9	58.1	1166-1337 bp	0.5	63.6
rrn23-trnA-UGC_p4	GAATCTCCGGATCCATGCTC	TTCCCGGCCTTCTTGGTATA	55.0	50.0	57.9	58.1	1167-1338 bp	0.5	63.6
rrn23-trnA-UGC_p5	TCATTTTCAGCTCCCCGAAG	TCCCGGCCTTCTTGGTATAA	50.0	50.0	57.9	58.1	1148-1319 bp	0.5	63.5