Hotspot

ndhD-ccsA

Genome · 135103-135351 · 249 bp

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Pi 0.0361

MarkerSeek score 52.80

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: Genome
length_bp: 249
variable_sites: 18
pi: 0.0361
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	AGTAGAATTCATTTTGTCTCACA	CCATCAGGAAAAGGTTTGCAC	30.4	47.6	54.0	58.0	334-342 bp	1.0	44.3
ndhD-ccsA_p2	AGTAGAATTCATTTTGTCTCACA	CAGGAAAAGGTTTGCACAGT	30.4	45.0	54.0	56.7	330-338 bp	1.0	44.3
ndhD-ccsA_p3	AGTAGAATTCATTTTGTCTCACAA	CCATCAGGAAAAGGTTTGCAC	29.2	47.6	54.7	58.0	334-342 bp	1.0	44.3
ndhD-ccsA_p4	AGTAGAATTCATTTTGTCTCACA	TCAGGAAAAGGTTTGCACAGT	30.4	42.9	54.0	58.3	331-339 bp	1.0	44.3
ndhD-ccsA_p5	AAGTAGAATTCATTTTGTCTCACA	CCATCAGGAAAAGGTTTGCAC	29.2	47.6	54.7	58.0	335-343 bp	1.0	44.2