Hotspot

ndhE-psaC

Genome · 132853-133196 · 344 bp

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Pi 0.0213

MarkerSeek score 67.10

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: Genome
length_bp: 344
variable_sites: 14
pi: 0.0213
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	AAGCAGCAATAGGATCAGCC	GCCTTACATCCATCCCAAGG	50.0	55.0	58.0	58.0	540 bp	1.0	81.8
ndhE-psaC_p2	AAGCAGCAATAGGATCAGCC	GGTAGGACAAGCTCTTACGC	50.0	55.0	58.0	58.1	502 bp	1.0	81.4
ndhE-psaC_p3	AAGCAGCAATAGGATCAGCC	CGCACTGGGTACAACCAATA	50.0	50.0	58.0	57.9	485 bp	1.0	81.3
ndhE-psaC_p4	GCAGCAATAGGATCAGCCAT	GCCTTACATCCATCCCAAGG	50.0	55.0	58.1	58.0	538 bp	1.0	81.2
ndhE-psaC_p5	AAGCAGCAATAGGATCAGCC	ACATCGGTAGGACAAGCTCT	50.0	50.0	58.0	58.1	507 bp	1.0	80.9