Hotspot

trnA-UGC-rrn23

Genome · 110680-111640 · 961 bp

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Pi 0.00821
MarkerSeek score 63.80
Primer available yes
Label trnA-UGC-rrn23

Feature Metadata

label_name
trnA-UGC-rrn23
feature_type
igs
region
Genome
length_bp
961
variable_sites
11
pi
0.00821
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.82
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC-rrn23_p1 TCCCGGCCTTCTTGGTATAA GTCTCTGGGTGCCTAGGTAT 50.0 55.0 58.1 57.9 1100-1271 bp 1.0 83.1
trnA-UGC-rrn23_p2 TTCCCGGCCTTCTTGGTATA GTCTCTGGGTGCCTAGGTAT 50.0 55.0 58.1 57.9 1101-1272 bp 1.0 83.1
trnA-UGC-rrn23_p3 TCCCGGCCTTCTTGGTATAA GAATCTCCGGATCCATGCTC 50.0 55.0 58.1 57.9 1166-1337 bp 1.0 83.0
trnA-UGC-rrn23_p4 TTCCCGGCCTTCTTGGTATA GAATCTCCGGATCCATGCTC 50.0 55.0 58.1 57.9 1167-1338 bp 1.0 83.0
trnA-UGC-rrn23_p5 TCCCGGCCTTCTTGGTATAA TCATTTTCAGCTCCCCGAAG 50.0 50.0 58.1 57.9 1148-1319 bp 1.0 82.9