Hotspot

rpoC1-rpoB

Genome · 93234-93289 · 56 bp

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Pi 0.0655

MarkerSeek score 76.80

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: Genome
length_bp: 56
variable_sites: 7
pi: 0.0655
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	CGCGAATCTGTTCAGGAGAA	AGATCATGCTGTTGTGAAAA	50.0	35.0	58.0	53.4	166-167 bp	1.0	46.5
rpoC1-rpoB_p2	CGCGAATCTGTTCAGGAGAA	AGATCATGCTGTTGTGAAAAA	50.0	33.3	58.0	54.3	166-167 bp	1.0	45.6
rpoC1-rpoB_p3	AGAAGCCAATCCAATGCGAA	AGATCATGCTGTTGTGAAAA	45.0	35.0	58.2	53.4	150-151 bp	1.0	45.5
rpoC1-rpoB_p4	AGGAGAAGCCAATCCAATGC	AGATCATGCTGTTGTGAAAA	50.0	35.0	58.2	53.4	153-154 bp	1.0	44.9
rpoC1-rpoB_p5	AGAAGCCAATCCAATGCGAA	AGATCATGCTGTTGTGAAAAA	45.0	33.3	58.2	54.3	150-151 bp	1.0	44.7