Hotspot

atpA-atpF

Genome · 80487-80531 · 45 bp

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Pi 0.0704

MarkerSeek score 72.70

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: Genome
length_bp: 45
variable_sites: 6
pi: 0.0704
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	GCTAATTTCCTCGGGTCGAA	CGCAGAAAACACAACCGATT	50.0	45.0	57.7	57.6	97-98 bp	1.0	79.3
atpA-atpF_p2	CCTCGGGTCGAATGTTTACC	CGCAGAAAACACAACCGATT	55.0	45.0	58.4	57.6	89-90 bp	1.0	79.3
atpA-atpF_p3	CTCGGGTCGAATGTTTACCA	CGCAGAAAACACAACCGATT	50.0	45.0	57.6	57.6	88-89 bp	1.0	79.2
atpA-atpF_p4	ACACTGCTAATTTCCTCGGG	CGCAGAAAACACAACCGATT	50.0	45.0	57.6	57.6	102-103 bp	1.0	78.3
atpA-atpF_p5	CACTGCTAATTTCCTCGGGT	CGCAGAAAACACAACCGATT	50.0	45.0	57.6	57.6	101-102 bp	1.0	78.3