Hotspot

trnG-UCC-trnR-UCU

Genome · 77251-78725 · 1475 bp

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Pi 0.0181
MarkerSeek score 61.90
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
Genome
length_bp
1475
variable_sites
52
pi
0.0181
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 ACCCTTAGCCTTCCAAGCTA CTGTCCTATCCATTAGACGATGG 50.0 47.8 58.0 57.9 1552-1553 bp 1.0 57.9
trnG-UCC-trnR-UCU_p2 ACCCTTAGCCTTCCAAGCTA TGTCCTATCCATTAGACGATGG 50.0 45.5 58.0 56.9 1551-1552 bp 1.0 57.3
trnG-UCC-trnR-UCU_p3 TCGACCATAACCCTTAGCCT CTGTCCTATCCATTAGACGATGG 50.0 47.8 57.8 57.9 1561-1562 bp 1.0 56.8
trnG-UCC-trnR-UCU_p4 GTCGACCATAACCCTTAGCC CTGTCCTATCCATTAGACGATGG 55.0 47.8 57.8 57.9 1562-1563 bp 1.0 56.3
trnG-UCC-trnR-UCU_p5 TCGACCATAACCCTTAGCCT TGTCCTATCCATTAGACGATGG 50.0 45.5 57.8 56.9 1560-1561 bp 1.0 56.2