Hotspot

trnfM-CAU-trnG-GCC

Genome · 52194-52320 · 127 bp

Back to Huperzia

Pi 0.0276

MarkerSeek score 63.40

Primer available yes

Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name: trnfM-CAU-trnG-GCC
feature_type: igs
region: Genome
length_bp: 127
variable_sites: 6
pi: 0.0276
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-GCC_p1	CTTGAGGTCACGGGTTCAAA	AGAAGATGCGGGTTCGATTC	50.0	50.0	58.0	58.1	193-194 bp	1.0	81.7
trnfM-CAU-trnG-GCC_p2	CTTGAGGTCACGGGTTCAAA	GAGAAGATGCGGGTTCGATT	50.0	50.0	58.0	58.1	194-195 bp	1.0	81.7
trnfM-CAU-trnG-GCC_p3	TTCATGAATTGCGCGGAGTA	AGAAGATGCGGGTTCGATTC	45.0	50.0	58.0	58.1	244-245 bp	1.0	81.4
trnfM-CAU-trnG-GCC_p4	TTCATGAATTGCGCGGAGTA	GAGAAGATGCGGGTTCGATT	45.0	50.0	58.0	58.1	245-246 bp	1.0	81.4
trnfM-CAU-trnG-GCC_p5	AACATTCATGAATTGCGCGG	AGAAGATGCGGGTTCGATTC	45.0	50.0	58.1	58.1	248-249 bp	1.0	80.8