Hotspot

rps4-trnS-GGA

Genome · 43362-43742 · 381 bp

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Pi 0.0232

MarkerSeek score 61.10

Primer available yes

Label rps4-trnS-GGA

Feature Metadata

label_name: rps4-trnS-GGA
feature_type: igs
region: Genome
length_bp: 381
variable_sites: 17
pi: 0.0232
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnS-GGA_p1	AACGAATTGCTAGTTGTGGA	CTCTTGCTTACCGAGGGTTC	40.0	55.0	55.3	58.0	458-460 bp	1.0	61.6
rps4-trnS-GGA_p2	ACGAATTGCTAGTTGTGGAA	CTCTTGCTTACCGAGGGTTC	40.0	55.0	55.3	58.0	457-459 bp	1.0	61.6
rps4-trnS-GGA_p3	AACGAATTGCTAGTTGTGGA	GGGTTCGAATCCCTCTCTCT	40.0	55.0	55.3	58.2	444-446 bp	1.0	60.1
rps4-trnS-GGA_p4	ACGAATTGCTAGTTGTGGAA	GGGTTCGAATCCCTCTCTCT	40.0	55.0	55.3	58.2	443-445 bp	1.0	60.1
rps4-trnS-GGA_p5	AACGAATTGCTAGTTGTGGAA	CTCTTGCTTACCGAGGGTTC	38.1	55.0	56.1	58.0	458-460 bp	1.0	60.0