Hotspot

rpl14-rps8

Genome · 5584-5964 · 381 bp

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Pi 0.0189

MarkerSeek score 62.30

Primer available yes

Label rpl14-rps8

Feature Metadata

label_name: rpl14-rps8
feature_type: igs
region: Genome
length_bp: 381
variable_sites: 14
pi: 0.0189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps8_p1	TTTTTGGTCCAGTTGCTCGA	GCAATGGTGTCGTTACCCAT	45.0	50.0	58.0	58.5	464-547 bp	1.0	80.0
rpl14-rps8_p2	CGAGTTTTTGGTCCAGTTGC	GCAATGGTGTCGTTACCCAT	50.0	50.0	57.9	58.5	468-551 bp	1.0	79.4
rpl14-rps8_p3	GTTTTTGGTCCAGTTGCTCG	GCAATGGTGTCGTTACCCAT	50.0	50.0	57.9	58.5	465-548 bp	1.0	79.4
rpl14-rps8_p4	TTTTTGGTCCAGTTGCTCGA	TTGGCAATGGTGTCGTTACC	45.0	50.0	58.0	58.8	467-550 bp	1.0	78.2
rpl14-rps8_p5	GTTTTTGGTCCAGTTGCTCG	TTGGCAATGGTGTCGTTACC	50.0	50.0	57.9	58.8	468-551 bp	1.0	77.6