Hotspot

rpl23-rpl2

Genome · 615-652 · 38 bp

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Pi 0.0614

MarkerSeek score 77.20

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: Genome
length_bp: 38
variable_sites: 4
pi: 0.0614
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	TGCGGGTTCAATAGTAACACA	GCGCCTGGAGTATAGTCTCT	42.9	55.0	57.6	58.4	170 bp	1.0	67.5
rpl23-rpl2_p2	TGCGGGTTCAATAGTAACACA	CAACTAATCGATCACGTGCG	42.9	50.0	57.6	57.4	187 bp	1.0	65.6
rpl23-rpl2_p3	CATCGACCCCCAGGAAAAA	GCGCCTGGAGTATAGTCTCT	52.6	55.0	57.3	58.4	196 bp	1.0	65.2
rpl23-rpl2_p4	TGCGGGTTCAATAGTAACACA	TGCGCCTGGAGTATAGTCTC	42.9	55.0	57.6	58.7	171 bp	1.0	65.2
rpl23-rpl2_p5	CATCGACCCCCAGGAAAAA	CAACTAATCGATCACGTGCG	52.6	50.0	57.3	57.4	213 bp	1.0	63.3