Hotspot

ycf2

IRa · 149352-156332 · 6981 bp

Back to Huodendron

Pi 0.000286

MarkerSeek score 37.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6981
variable_sites: 2
pi: 0.000286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CCAGCGACGGTTTCATTAGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	725-1103 bp	1.0	80.9
ycf2_p2	TCCAGCGACGGTTTCATTAG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	726-1104 bp	1.0	80.9
ycf2_p3	CCAGCGACGGTTTCATTAGA	CCACAACGATGCTGTCCATA	50.0	50.0	58.0	58.0	1345-1723 bp	1.0	80.5
ycf2_p4	TCCAGCGACGGTTTCATTAG	CCACAACGATGCTGTCCATA	50.0	50.0	58.0	58.0	1346-1724 bp	1.0	80.5
ycf2_p5	CCAGCGACGGTTTCATTAGA	TTCTACCCTTTCCCGGAAGT	50.0	50.0	58.0	58.0	1113-1491 bp	1.0	80.5