Hotspot

rps16

LSC · 5217-6343 · 1127 bp

Back to Huodendron

Pi 0

MarkerSeek score 59.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1127
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CGAGTCATCACTACCCCACT	CAATTCCATACCGGCTCCAA	55.0	50.0	58.5	57.9	1188-1193 bp	1.0	75.7
rps16_p2	TACGAGTCATCACTACCCCA	CAATTCCATACCGGCTCCAA	50.0	50.0	57.2	57.9	1190-1195 bp	1.0	73.4
rps16_p3	ACGAGTCATCACTACCCCAC	CAATTCCATACCGGCTCCAA	55.0	50.0	58.8	57.9	1189-1194 bp	1.0	73.4
rps16_p4	CGAGTCATCACTACCCCACT	ATCAATTCCATACCGGCTCC	55.0	50.0	58.5	57.4	1190-1195 bp	1.0	71.6
rps16_p5	CGAGTCATCACTACCCCACT	TCAATTCCATACCGGCTCCA	55.0	50.0	58.5	58.8	1189-1194 bp	1.0	69.8