Hotspot

rpoC2

LSC · 18079-22212 · 4134 bp

Back to Huodendron

Pi 0.000484

MarkerSeek score 54.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4134
variable_sites: 2
pi: 0.000484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCCGAGGACTCACAGAACT	GATCCCCAAGGCCAAATGAT	50.0	50.0	58.0	57.9	233 bp	1.0	79.9
rpoC2_p2	ATTCTTTCCGGCATCATCCC	GATCCCCAAGGCCAAATGAT	50.0	50.0	58.0	57.9	254 bp	1.0	79.8
rpoC2_p3	AGGACTCACAGAACTACCCC	GATCCCCAAGGCCAAATGAT	55.0	50.0	58.1	57.9	228 bp	1.0	79.5
rpoC2_p4	ATCATCCCATTCCGAGGACT	GATCCCCAAGGCCAAATGAT	50.0	50.0	57.9	57.9	242 bp	1.0	79.0
rpoC2_p5	CATCATCCCATTCCGAGGAC	GATCCCCAAGGCCAAATGAT	55.0	50.0	57.8	57.9	243 bp	1.0	78.5