Hotspot

ccsA

SSC · 118088-119047 · 960 bp

Back to Huodendron

Pi 0.00208

MarkerSeek score 59.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 2
pi: 0.00208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATGCTATCTTCTAA	ATTCACATGGTTCTCGCTGG	50.0	50.0	57.8	58.3	1141 bp	1.0	76.7
ccsA_p2	GGCGGCATGCTATCTTCTAA	TGATTCACATGGTTCTCGCT	50.0	45.0	57.8	57.2	1143 bp	1.0	72.6
ccsA_p3	GGCGGCATGCTATCTTCTAA	GTGATTCACATGGTTCTCGC	50.0	50.0	57.8	57.2	1144 bp	1.0	72.3
ccsA_p4	GGCGGCATGCTATCTTCTAA	CATGGTTCTCGCTGGCTTAC	50.0	55.0	57.8	59.0	1136 bp	1.0	70.9
ccsA_p5	TGGCGGCATGCTATCTTCTA	ATTCACATGGTTCTCGCTGG	50.0	50.0	59.0	58.3	1142 bp	1.0	70.5