Hotspot

accD

LSC · 60421-62619 · 2199 bp

Back to Huodendron

Pi 0.00324

MarkerSeek score 60.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 2199
variable_sites: 7
pi: 0.00324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCTACAAAAGAGAATCCTTTTT	ACGTCACCAAAGAAAACCCC	31.8	50.0	52.3	58.6	2416-2479 bp	1.0	41.4
accD_p2	CCTACAAAAGAGAATCCTTTTT	CGTCACCAAAGAAAACCCCA	31.8	50.0	52.3	58.6	2415-2478 bp	1.0	41.4
accD_p3	CCTACAAAAGAGAATCCTTTTT	TCACCAAAGAAAACCCCACT	31.8	45.0	52.3	57.2	2413-2476 bp	1.0	41.4
accD_p4	CCTACAAAAGAGAATCCTTTTT	GTCACCAAAGAAAACCCCAC	31.8	50.0	52.3	57.1	2414-2477 bp	1.0	41.4
accD_p5	CCTACAAAAGAGAATCCTTTTT	ACCAAAGAAAACCCCACTCT	31.8	45.0	52.3	56.9	2411-2474 bp	1.0	41.4