Hotspot

ycf2-trnI-CAU

IRa · 156333-156420 · 88 bp

Back to Huodendron

Pi 0.114
MarkerSeek score 72.70
Primer available yes
Label ycf2-trnI-CAU

Feature Metadata

label_name
ycf2-trnI-CAU
feature_type
igs
region
IRa
length_bp
88
variable_sites
10
pi
0.114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnI-CAU_p1 CCAGCGACGGTTTCATTAGA GTTGGGCGCTTTAACCATTC 50.0 50.0 58.0 58.0 725-1103 bp 1.0 80.9
ycf2-trnI-CAU_p2 TCCAGCGACGGTTTCATTAG GTTGGGCGCTTTAACCATTC 50.0 50.0 58.0 58.0 726-1104 bp 1.0 80.9
ycf2-trnI-CAU_p3 CCAGCGACGGTTTCATTAGA CCACAACGATGCTGTCCATA 50.0 50.0 58.0 58.0 1345-1723 bp 1.0 80.5
ycf2-trnI-CAU_p4 TCCAGCGACGGTTTCATTAG CCACAACGATGCTGTCCATA 50.0 50.0 58.0 58.0 1346-1724 bp 1.0 80.5
ycf2-trnI-CAU_p5 CCAGCGACGGTTTCATTAGA TTCTACCCTTTCCCGGAAGT 50.0 50.0 58.0 58.0 1113-1491 bp 1.0 80.5