Hotspot

trnI-CAU-ycf2

IRb · 89811-89898 · 88 bp

Back to Huodendron

Pi 0.114
MarkerSeek score 72.70
Primer available yes
Label trnI-CAU-ycf2

Feature Metadata

label_name
trnI-CAU-ycf2
feature_type
igs
region
IRb
length_bp
88
variable_sites
10
pi
0.114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC CCAGCGACGGTTTCATTAGA 50.0 50.0 58.0 58.0 725-1103 bp 1.0 80.9
trnI-CAU-ycf2_p2 GTTGGGCGCTTTAACCATTC TCCAGCGACGGTTTCATTAG 50.0 50.0 58.0 58.0 726-1104 bp 1.0 80.9
trnI-CAU-ycf2_p3 CCACAACGATGCTGTCCATA CCAGCGACGGTTTCATTAGA 50.0 50.0 58.0 58.0 1345-1723 bp 1.0 80.5
trnI-CAU-ycf2_p4 CCACAACGATGCTGTCCATA TCCAGCGACGGTTTCATTAG 50.0 50.0 58.0 58.0 1346-1724 bp 1.0 80.5
trnI-CAU-ycf2_p5 TTCTACCCTTTCCCGGAAGT CCAGCGACGGTTTCATTAGA 50.0 50.0 58.0 58.0 1113-1491 bp 1.0 80.5