Hotspot

matK-trnK-UUU

LSC · 3606-4321 · 716 bp

Back to Huodendron

Pi 0.00279

MarkerSeek score 60.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 2
pi: 0.00279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCGTGTTGCTGAGATCTACC	CGATTCGGGTTGCTAACTCA	50.0	50.0	57.1	58.0	809 bp	1.0	72.6
matK-trnK-UUU_p2	CGTGTTGCTGAGATCTACCT	CGATTCGGGTTGCTAACTCA	50.0	50.0	57.1	58.0	808 bp	1.0	72.6
matK-trnK-UUU_p3	TCGTGTTGCTGAGATCTACC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.1	58.2	790 bp	1.0	71.3
matK-trnK-UUU_p4	TCGTGTTGCTGAGATCTACC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.1	58.2	789 bp	1.0	71.3
matK-trnK-UUU_p5	CGTGTTGCTGAGATCTACCT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.1	58.2	788 bp	1.0	71.3