primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	AAAGGAGCAATAACGCCCTC	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.267306	491	1552	1209-1209	1.000000	77.9
psbA_p2	psbA	2	AGCAATAACGCCCTCTTCTC	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.336273	491	1552	1204-1204	1.000000	77.3
psbA_p3	psbA	3	GAGCAATAACGCCCTCTTCT	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.336273	491	1552	1205-1205	1.000000	77.3
psbA_p4	psbA	4	GCAATAACGCCCTCTTCTCT	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.336273	491	1552	1203-1203	1.000000	77.3
psbA_p5	psbA	5	GGAGCAATAACGCCCTCTTC	AAATTCGTGTGCTTGGGAGT	20	20	55.0	45.0	58.7	58.0	0.0	0.0	0.718262	491	1552	1206-1206	1.000000	74.3
trnK-UUU_p1	trnK-UUU	1	GCCCGTGTCAACCAATATCT	TCTGATTGTATCTACACGCCT	20	21	50.0	42.9	58.0	56.3	0.0	0.0	2.767044	1772	4358	2740-2740	1.000000	57.9
trnK-UUU_p2	trnK-UUU	2	TGCCCGTGTCAACCAATATC	TCTGATTGTATCTACACGCCT	20	21	50.0	42.9	58.3	56.3	0.0	0.0	2.983425	1772	4358	2741-2741	1.000000	56.2
trnK-UUU_p3	trnK-UUU	3	GCCCGTGTCAACCAATATCT	TCTGATTGTATCTACACGCCTT	20	22	50.0	40.9	58.0	57.0	0.0	0.0	3.040830	1772	4358	2740-2740	1.000000	55.7
trnK-UUU_p4	trnK-UUU	4	GCCCGTGTCAACCAATATCT	TTCTGATTGTATCTACACGCCT	20	22	50.0	40.9	58.0	57.0	0.0	0.0	3.040830	1772	4358	2741-2741	1.000000	55.7
trnK-UUU_p5	trnK-UUU	5	ATGCCCGTGTCAACCAATAT	TCTGATTGTATCTACACGCCT	20	21	45.0	42.9	57.6	56.3	0.0	0.0	3.164030	1772	4358	2742-2742	1.000000	54.7
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCGTGTTGCTGAGATCTACC	CGATTCGGGTTGCTAACTCA	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.946035	3606	4321	809-809	1.000000	72.6
matK-trnK-UUU_p2	matK-trnK-UUU	2	CGTGTTGCTGAGATCTACCT	CGATTCGGGTTGCTAACTCA	20	20	50.0	50.0	57.1	58.0	0.0	0.0	0.954034	3606	4321	808-808	1.000000	72.6
matK-trnK-UUU_p3	matK-trnK-UUU	3	TCGTGTTGCTGAGATCTACC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.1	58.2	0.0	17.1	1.109873	3606	4321	790-790	1.000000	71.3
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCGTGTTGCTGAGATCTACC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.1	58.2	0.0	17.1	1.109873	3606	4321	789-789	1.000000	71.3
matK-trnK-UUU_p5	matK-trnK-UUU	5	CGTGTTGCTGAGATCTACCT	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.1	58.2	0.0	17.1	1.117871	3606	4321	788-788	1.000000	71.3
rps16_p1	rps16	1	CGAGTCATCACTACCCCACT	CAATTCCATACCGGCTCCAA	20	20	55.0	50.0	58.5	57.9	0.0	0.0	0.575932	5217	6343	1188-1193	1.000000	75.7
rps16_p2	rps16	2	TACGAGTCATCACTACCCCA	CAATTCCATACCGGCTCCAA	20	20	50.0	50.0	57.2	57.9	0.0	0.0	0.864171	5217	6343	1190-1195	1.000000	73.4
rps16_p3	rps16	3	ACGAGTCATCACTACCCCAC	CAATTCCATACCGGCTCCAA	20	20	55.0	50.0	58.8	57.9	0.0	0.0	0.867786	5217	6343	1189-1194	1.000000	73.4
rps16_p4	rps16	4	CGAGTCATCACTACCCCACT	ATCAATTCCATACCGGCTCC	20	20	55.0	50.0	58.5	57.4	0.0	0.0	1.093220	5217	6343	1190-1195	1.000000	71.6
rps16_p5	rps16	5	CGAGTCATCACTACCCCACT	TCAATTCCATACCGGCTCCA	20	20	55.0	50.0	58.5	58.8	0.0	0.0	1.315892	5217	6343	1189-1194	1.000000	69.8
rpoC2_p1	rpoC2	1	TTCCGAGGACTCACAGAACT	GATCCCCAAGGCCAAATGAT	20	20	50.0	50.0	58.0	57.9	0.0	5.0	0.095800	21599	21718	233-233	1.000000	79.9
rpoC2_p2	rpoC2	2	ATTCTTTCCGGCATCATCCC	GATCCCCAAGGCCAAATGAT	20	20	50.0	50.0	58.0	57.9	0.0	5.0	0.105716	21599	21718	254-254	1.000000	79.8
rpoC2_p3	rpoC2	3	AGGACTCACAGAACTACCCC	GATCCCCAAGGCCAAATGAT	20	20	55.0	50.0	58.1	57.9	0.0	5.0	0.152618	21599	21718	228-228	1.000000	79.5
rpoC2_p4	rpoC2	4	ATCATCCCATTCCGAGGACT	GATCCCCAAGGCCAAATGAT	20	20	50.0	50.0	57.9	57.9	0.0	5.0	0.201827	21599	21718	242-242	1.000000	79.0
rpoC2_p5	rpoC2	5	CATCATCCCATTCCGAGGAC	GATCCCCAAGGCCAAATGAT	20	20	55.0	50.0	57.8	57.9	0.0	5.0	0.268476	21599	21718	243-243	1.000000	78.5
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGGGACTGCAAATCCTTTT	GCCCAAGCGAGACTTACAAT	20	20	50.0	50.0	58.4	58.3	14.7	0.0	0.616424	29662	30233	655-655	1.000000	75.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	29662	30233	717-717	1.000000	74.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	AGTTCAAATCTGGGTGTCGC	GCCCAAGCGAGACTTACAAT	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.737176	29662	30233	630-630	1.000000	74.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.5	57.6	0.0	0.0	0.827105	29662	30233	692-692	1.000000	73.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	TATCCTTTTGGCGACATGGC	GCCCAAGCGAGACTTACAAT	20	20	50.0	50.0	58.6	58.3	0.0	0.0	0.872387	29662	30233	688-688	1.000000	73.1
trnD-GUC_p1	trnD-GUC	1	TGGTGAGAAACGCGAAAGAA	ATTCGCCAATCTACCACGAG	20	20	45.0	50.0	58.1	57.8	15.9	0.0	0.282391	32611	32684	294-295	1.000000	78.0
trnD-GUC_p2	trnD-GUC	2	CTGTGAAATCCTGTCCCCTG	ATTCGCCAATCTACCACGAG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.344870	32611	32684	247-247	1.000000	77.6
trnD-GUC_p3	trnD-GUC	3	TGGTGAGAAACGCGAAAGAA	GGCCCAATAATTCGCCAATC	20	20	45.0	50.0	58.1	57.6	15.9	0.0	0.496191	32611	32684	303-304	1.000000	76.3
trnD-GUC_p4	trnD-GUC	4	CTGTGAAATCCTGTCCCCTG	GGCCCAATAATTCGCCAATC	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.558670	32611	32684	256-256	1.000000	75.8
trnD-GUC_p5	trnD-GUC	5	TGGTGAGAAACGCGAAAGAA	CGCCAATCTACCACGAGATG	20	20	45.0	55.0	58.1	58.5	15.9	0.0	0.565653	32611	32684	291-292	1.000000	75.7
trnF-GAA_p1	trnF-GAA	1	GAGAATGATGCGTCGGGAAT	TCTCTAAACACCTAGATAGATGGC	20	24	50.0	41.7	58.1	57.1	0.6	0.0	5.031691	50870	50942	213-213	1.000000	40.4
trnF-GAA_p2	trnF-GAA	2	GAGAATGATGCGTCGGGAAT	CTCTAAACACCTAGATAGATGGCT	20	24	50.0	41.7	58.1	57.1	0.6	0.0	5.038470	50870	50942	212-212	1.000000	40.4
trnF-GAA_p3	trnF-GAA	3	GAGAATGATGCGTCGGGAAT	AAACACCTAGATAGATGGCT	20	20	50.0	40.0	58.1	53.1	0.6	0.0	5.042127	50870	50942	208-208	1.000000	40.4
trnF-GAA_p4	trnF-GAA	4	CCAGGACCCGGCTAAAATTT	TCTCTAAACACCTAGATAGATGGC	20	24	50.0	41.7	58.2	57.1	0.0	0.0	5.055443	50870	50942	287-287	1.000000	40.3
trnF-GAA_p5	trnF-GAA	5	CCAGGACCCGGCTAAAATTT	CTCTAAACACCTAGATAGATGGCT	20	24	50.0	41.7	58.2	57.1	0.0	0.0	5.062222	50870	50942	286-286	1.000000	40.3
accD_p1	accD	1	CCTACAAAAGAGAATCCTTTTT	ACGTCACCAAAGAAAACCCC	22	20	31.8	50.0	52.3	58.6	6.7	0.0	8.321770	60421	62619	2416-2479	1.000000	41.4
accD_p2	accD	2	CCTACAAAAGAGAATCCTTTTT	CGTCACCAAAGAAAACCCCA	22	20	31.8	50.0	52.3	58.6	6.7	0.0	8.322787	60421	62619	2415-2478	1.000000	41.4
accD_p3	accD	3	CCTACAAAAGAGAATCCTTTTT	TCACCAAAGAAAACCCCACT	22	20	31.8	45.0	52.3	57.2	6.7	0.0	8.556042	60421	62619	2413-2476	1.000000	41.4
accD_p4	accD	4	CCTACAAAAGAGAATCCTTTTT	GTCACCAAAGAAAACCCCAC	22	20	31.8	50.0	52.3	57.1	6.7	0.0	8.600332	60421	62619	2414-2477	1.000000	41.4
accD_p5	accD	5	CCTACAAAAGAGAATCCTTTTT	ACCAAAGAAAACCCCACTCT	22	20	31.8	45.0	52.3	56.9	6.7	0.0	8.862932	60421	62619	2411-2474	1.000000	41.4
accD-psaI_p1	accD-psaI	1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.8	58.0	0.0	26.9	0.787656	62620	63394	879-879	1.000000	73.8
accD-psaI_p2	accD-psaI	2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.8	57.9	0.0	26.8	0.864190	62620	63394	885-885	1.000000	73.2
accD-psaI_p3	accD-psaI	3	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	19	20	52.6	45.0	58.1	58.0	0.0	26.9	1.092920	62620	63394	879-879	1.000000	71.3
accD-psaI_p4	accD-psaI	4	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	58.1	58.0	0.0	26.9	1.101279	62620	63394	877-877	1.000000	71.3
accD-psaI_p5	accD-psaI	5	TTGATCTAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	56.9	58.0	4.9	26.9	1.168486	62620	63394	934-934	1.000000	70.7
rpl23_p1	rpl23	1	TTTCACTTGACTGCCTACGG	ACATAACGAATTGGTGTGGT	20	20	50.0	40.0	57.8	55.3	0.0	9.8	2.898410	89290	89571	468-468	1.000000	56.8
rpl23_p2	rpl23	2	GTGTGCTCGGGTTAGAAGTT	ACATAACGAATTGGTGTGGT	20	20	50.0	40.0	57.8	55.3	0.0	9.8	2.899910	89290	89571	439-439	1.000000	56.8
rpl23_p3	rpl23	3	CACTTGACTGCCTACGGTTC	ACATAACGAATTGGTGTGGT	20	20	55.0	40.0	58.6	55.3	0.0	9.8	3.305129	89290	89571	465-465	1.000000	53.6
rpl23_p4	rpl23	4	TTTCACTTGACTGCCTACGG	CCATACATAACGAATTGGTGTGG	20	23	50.0	43.5	57.8	57.8	0.0	9.8	3.332344	89290	89571	472-472	1.000000	53.3
rpl23_p5	rpl23	5	GTGTGCTCGGGTTAGAAGTT	CCATACATAACGAATTGGTGTGG	20	23	50.0	43.5	57.8	57.8	0.0	9.8	3.333845	89290	89571	443-443	1.000000	53.3
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.013783	89811	89898	725-1103	1.000000	80.9
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	TCCAGCGACGGTTTCATTAG	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.013783	89811	89898	726-1104	1.000000	80.9
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	CCACAACGATGCTGTCCATA	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027347	89811	89898	1345-1723	1.000000	80.5
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	CCACAACGATGCTGTCCATA	TCCAGCGACGGTTTCATTAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027347	89811	89898	1346-1724	1.000000	80.5
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	TTCTACCCTTTCCCGGAAGT	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.039019	89811	89898	1113-1491	1.000000	80.5
rps7-rps12_p1	rps7-rps12	1	TCTTTTGCAGTACCTCGACG	CTGTGGAAAGCCGTATTCGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.064908	100832	100884	184-184	1.000000	79.9
rps7-rps12_p2	rps7-rps12	2	TCTTTTGCAGTACCTCGACG	TCTGTGGAAAGCCGTATTCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.064908	100832	100884	185-185	1.000000	79.9
rps7-rps12_p3	rps7-rps12	3	CTTTTGCAGTACCTCGACGT	CTGTGGAAAGCCGTATTCGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.225013	100832	100884	183-183	1.000000	78.6
rps7-rps12_p4	rps7-rps12	4	CTTTTGCAGTACCTCGACGT	TCTGTGGAAAGCCGTATTCG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.225013	100832	100884	184-184	1.000000	78.6
rps7-rps12_p5	rps7-rps12	5	TCTTTTGCAGTACCTCGACG	GGCTTGGAGGGAGATCTTTC	20	20	50.0	55.0	57.9	57.7	0.0	0.0	0.400536	100832	100884	148-148	1.000000	77.3
ndhF-rpl32_p1	ndhF-rpl32	1	ACAAAAGGTATGATCCACGCA	AGCTGCCCAATATCCTTTCT	21	20	42.9	45.0	57.9	56.6	0.0	0.0	2.504112	115875	116836	1087-1090	1.000000	60.1
ndhF-rpl32_p2	ndhF-rpl32	2	ACAAAAGGTATGATCCACGCA	AAGCTGCCCAATATCCTTTCT	21	21	42.9	42.9	57.9	57.3	0.0	0.0	2.743447	115875	116836	1088-1091	1.000000	58.2
ndhF-rpl32_p3	ndhF-rpl32	3	ACAAAAGGTATGATCCACGCA	AGCTGCCCAATATCCTTTCTT	21	21	42.9	42.9	57.9	57.3	0.0	0.0	2.743447	115875	116836	1087-1090	1.000000	58.2
ndhF-rpl32_p4	ndhF-rpl32	4	ACAAAAGGTATGATCCACGCA	AAGCTGCCCAATATCCTTTCTT	21	22	42.9	40.9	57.9	58.0	0.0	0.0	3.079856	115875	116836	1088-1091	1.000000	55.5
ndhF-rpl32_p5	ndhF-rpl32	5	ACAAAAGGTATGATCCACGCA	AGCTGCCCAATATCCTTTCTTT	21	22	42.9	40.9	57.9	58.0	0.0	0.0	3.079856	115875	116836	1087-1090	1.000000	55.5
ccsA_p1	ccsA	1	GGCGGCATGCTATCTTCTAA	ATTCACATGGTTCTCGCTGG	20	20	50.0	50.0	57.8	58.3	18.9	0.0	0.427071	118088	119047	1141-1141	1.000000	76.7
ccsA_p2	ccsA	2	GGCGGCATGCTATCTTCTAA	TGATTCACATGGTTCTCGCT	20	20	50.0	45.0	57.8	57.2	18.9	0.0	0.938085	118088	119047	1143-1143	1.000000	72.6
ccsA_p3	ccsA	3	GGCGGCATGCTATCTTCTAA	GTGATTCACATGGTTCTCGC	20	20	50.0	50.0	57.8	57.2	18.9	11.9	0.983611	118088	119047	1144-1144	1.000000	72.3
ccsA_p4	ccsA	4	GGCGGCATGCTATCTTCTAA	CATGGTTCTCGCTGGCTTAC	20	20	50.0	55.0	57.8	59.0	18.9	0.0	1.160003	118088	119047	1136-1136	1.000000	70.9
ccsA_p5	ccsA	5	TGGCGGCATGCTATCTTCTA	ATTCACATGGTTCTCGCTGG	20	20	50.0	50.0	59.0	58.3	18.9	0.0	1.209438	118088	119047	1142-1142	1.000000	70.5
rps12-rps7_p1	rps12-rps7	1	CTGTGGAAAGCCGTATTCGA	TCTTTTGCAGTACCTCGACG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.064908	145347	145399	184-184	1.000000	79.9
rps12-rps7_p2	rps12-rps7	2	TCTGTGGAAAGCCGTATTCG	TCTTTTGCAGTACCTCGACG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.064908	145347	145399	185-185	1.000000	79.9
rps12-rps7_p3	rps12-rps7	3	CTGTGGAAAGCCGTATTCGA	CTTTTGCAGTACCTCGACGT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.225013	145347	145399	183-183	1.000000	78.6
rps12-rps7_p4	rps12-rps7	4	TCTGTGGAAAGCCGTATTCG	CTTTTGCAGTACCTCGACGT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.225013	145347	145399	184-184	1.000000	78.6
rps12-rps7_p5	rps12-rps7	5	GGCTTGGAGGGAGATCTTTC	TCTTTTGCAGTACCTCGACG	20	20	55.0	50.0	57.7	57.9	0.0	0.0	0.400536	145347	145399	148-148	1.000000	77.3
ycf2_p1	ycf2	1	CCAGCGACGGTTTCATTAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.013783	156210	156329	725-1103	1.000000	80.9
ycf2_p2	ycf2	2	TCCAGCGACGGTTTCATTAG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.013783	156210	156329	726-1104	1.000000	80.9
ycf2_p3	ycf2	3	CCAGCGACGGTTTCATTAGA	CCACAACGATGCTGTCCATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027347	156210	156329	1345-1723	1.000000	80.5
ycf2_p4	ycf2	4	TCCAGCGACGGTTTCATTAG	CCACAACGATGCTGTCCATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027347	156210	156329	1346-1724	1.000000	80.5
ycf2_p5	ycf2	5	CCAGCGACGGTTTCATTAGA	TTCTACCCTTTCCCGGAAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.7	0.039019	156210	156329	1113-1491	1.000000	80.5
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	CCAGCGACGGTTTCATTAGA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.013783	156333	156420	725-1103	1.000000	80.9
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	TCCAGCGACGGTTTCATTAG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.013783	156333	156420	726-1104	1.000000	80.9
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CCAGCGACGGTTTCATTAGA	CCACAACGATGCTGTCCATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027347	156333	156420	1345-1723	1.000000	80.5
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	TCCAGCGACGGTTTCATTAG	CCACAACGATGCTGTCCATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027347	156333	156420	1346-1724	1.000000	80.5
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CCAGCGACGGTTTCATTAGA	TTCTACCCTTTCCCGGAAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.7	0.039019	156333	156420	1113-1491	1.000000	80.5