Hotspot

ycf1

SSC · 123149-128731 · 5583 bp

Back to Humulus

Pi 0.00341

MarkerSeek score 54.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5583
variable_sites: 19
pi: 0.00341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGCAATTTCATTTTTGATTGAAAGT	TGTTCACAACAGAGAACCCC	26.9	50.0	57.8	57.7	291 bp	1.0	40.8
ycf1_p2	TGGCAATTTCATTTTTGATTGAAAGT	GTTCACAACAGAGAACCCCC	26.9	55.0	57.8	58.4	290 bp	1.0	40.8
ycf1_p3	TGGCAATTTCATTTTTGATTGA	TGTTCACAACAGAGAACCCC	27.3	50.0	53.7	57.7	291 bp	1.0	40.8
ycf1_p4	TGGCAATTTCATTTTTGATTGA	GTTCACAACAGAGAACCCCC	27.3	55.0	53.7	58.4	290 bp	1.0	40.8
ycf1_p5	TGGCAATTTCATTTTTGATTGAA	TGTTCACAACAGAGAACCCC	26.1	50.0	54.5	57.7	291 bp	1.0	40.8