Hotspot

rpl16

LSC · 81215-82663 · 1449 bp

Back to Humulus

Pi 0.00278

MarkerSeek score 60.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1449
variable_sites: 4
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTCAATGCAAAGCAAAGGGC	TCCGTTGATTTAATATAATTGCT	45.0	26.1	58.1	52.3	1582-1591 bp	1.0	40.7
rpl16_p2	TTCAATGCAAAGCAAAGGGC	ATCCGTTGATTTAATATAATTGCT	45.0	25.0	58.1	53.0	1583-1592 bp	1.0	40.7
rpl16_p3	TTCAATGCAAAGCAAAGGGC	AATCCGTTGATTTAATATAATTGC	45.0	25.0	58.1	52.3	1584-1593 bp	1.0	40.7
rpl16_p4	TTCAATGCAAAGCAAAGGGC	GGTTGAATAAAAATTCTATTAATCCGT	45.0	25.9	58.1	55.1	1604-1613 bp	1.0	40.6
rpl16_p5	TTCAATGCAAAGCAAAGGGC	AGGTTGAATAAAAATTCTATTAATCCG	45.0	25.9	58.1	54.9	1605-1614 bp	1.0	40.6