Hotspot

atpF

LSC · 11921-13224 · 1304 bp

Back to Humulus

Pi 0.00537

MarkerSeek score 54.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1304
variable_sites: 7
pi: 0.00537
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCATCGGCTCGAATGGTT	TCTATAAGAAAGAGGAGACCA	45.0	38.1	57.9	52.4	1417-1420 bp	1.0	40.6
atpF_p2	TCATCGGCTCGAATGGTTAC	TCTATAAGAAAGAGGAGACCA	50.0	38.1	57.8	52.4	1415-1418 bp	1.0	40.6
atpF_p3	GGCTCGAATGGTTACCATGA	TCTATAAGAAAGAGGAGACCA	50.0	38.1	57.7	52.4	1410-1413 bp	1.0	40.6
atpF_p4	ATTTCATCGGCTCGAATGGT	TCTATAAGAAAGAGGAGACCA	45.0	38.1	57.7	52.4	1418-1421 bp	1.0	40.6
atpF_p5	TTTCATCGGCTCGAATGGTT	TCTATAAGAAAGAGGAGACCAT	45.0	36.4	57.9	53.1	1417-1420 bp	1.0	40.6