Hotspot

psbE-petL

LSC · 64842-65958 · 1117 bp

Back to Humulus

Pi 0.00717

MarkerSeek score 63.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 8
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1263-1268 bp	1.0	66.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1186-1191 bp	1.0	65.7
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1272-1277 bp	1.0	65.6
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1277-1282 bp	1.0	65.5
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1271-1276 bp	1.0	65.4