Hotspot

petA-psbJ

LSC · 63165-64063 · 899 bp

Back to Humulus

Pi 0.00336

MarkerSeek score 61.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 3
pi: 0.00336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACAAGGCCTTTTGCTCTTCT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1074-1080 bp	1.0	68.9
petA-psbJ_p2	TCCATTACGTGTACAAGGCC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.6	58.1	1086-1092 bp	1.0	68.8
petA-psbJ_p3	CCATTACGTGTACAAGGCCT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.6	58.1	1085-1091 bp	1.0	68.7
petA-psbJ_p4	ACAAGGCCTTTTGCTCTTCT	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.6	57.8	1075-1081 bp	1.0	68.2
petA-psbJ_p5	TCCATTACGTGTACAAGGCC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.6	57.8	1087-1093 bp	1.0	68.1