Hotspot

ycf4-cemA

LSC · 60585-61287 · 703 bp

Back to Humulus

Pi 0.00996

MarkerSeek score 64.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 7
pi: 0.00996
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CAGAAAGCTGCTGAATTGGC	AGAGAGATCCACCAAGGCAA	50.0	50.0	58.0	58.3	831 bp	1.0	77.9
ycf4-cemA_p2	GCCTTTTTCTTGCGTGTACC	AGAGAGATCCACCAAGGCAA	50.0	50.0	58.0	58.3	813 bp	1.0	77.6
ycf4-cemA_p3	GGCCTTTTTCTTGCGTGTAC	AGAGAGATCCACCAAGGCAA	50.0	50.0	58.0	58.3	814 bp	1.0	77.6
ycf4-cemA_p4	CATTCCCTTGACTCGTACCG	AGAGAGATCCACCAAGGCAA	55.0	50.0	58.1	58.3	880 bp	1.0	77.3
ycf4-cemA_p5	ATTGGCCTTTTTCTTGCGTG	AGAGAGATCCACCAAGGCAA	45.0	50.0	57.8	58.3	817 bp	1.0	76.8