Hotspot

accD-psaI

LSC · 58578-59467 · 890 bp

Back to Humulus

Pi 0.00457

MarkerSeek score 63.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 4
pi: 0.00457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGCGTTCTGGGTGAGTTATT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1021-1029 bp	1.0	80.4
accD-psaI_p2	GGCGTTCTGGGTGAGTTATT	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.9	58.0	1012-1020 bp	1.0	80.2
accD-psaI_p3	GGCGTTCTGGGTGAGTTATT	AGAAACCATTGCAATTGCCG	50.0	45.0	57.9	57.9	1026-1034 bp	1.0	79.9
accD-psaI_p4	TAAAAGGCGTTCTGGGTGAG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1026-1034 bp	1.0	79.8
accD-psaI_p5	TAAAAGGCGTTCTGGGTGAG	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.8	58.0	1017-1025 bp	1.0	79.7