Hotspot

psbC-trnS-UGA

LSC · 35461-35692 · 232 bp

Back to Humulus

Pi 0.0302

MarkerSeek score 56.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 7
pi: 0.0302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TGTCTCTCCGAGAAGTTGGT	ACCTAGAACGACCAGGTTCA	50.0	50.0	58.0	58.0	962-963 bp	1.0	80.7
psbC-trnS-UGA_p2	CTGCAGCGGGATTTGAAAAG	ACCTAGAACGACCAGGTTCA	50.0	50.0	58.0	58.0	867-868 bp	1.0	80.7
psbC-trnS-UGA_p3	TGTCTCTCCGAGAAGTTGGT	CGACCAACCATCAGGAGAAG	50.0	55.0	58.0	58.0	858-859 bp	1.0	80.7
psbC-trnS-UGA_p4	TCTAATGCGTTCACCGACAG	ACCTAGAACGACCAGGTTCA	50.0	50.0	58.0	58.0	1199-1200 bp	1.0	80.5
psbC-trnS-UGA_p5	TCTAATGCGTTCACCGACAG	CGACCAACCATCAGGAGAAG	50.0	55.0	58.0	58.0	1095-1096 bp	1.0	80.5