Hotspot

trnC-GCA-petN

LSC · 28010-28943 · 934 bp

Back to Humulus

Pi 0.00642

MarkerSeek score 61.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 6
pi: 0.00642
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	990-992 bp	1.0	78.6
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1042-1044 bp	1.0	78.1
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1051-1053 bp	1.0	77.0
trnC-GCA-petN_p4	GTATCATTTTGGCGGCATGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	1103-1105 bp	1.0	76.5
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1008-1010 bp	1.0	76.4