Hotspot

rpoB-trnC-GCA

LSC · 26718-27938 · 1221 bp

Back to Humulus

Pi 0.00493

MarkerSeek score 62.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 6
pi: 0.00493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCATTTCCATCCCCAAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1299-1303 bp	1.0	78.4
rpoB-trnC-GCA_p2	CCATTTCCATCCCCAAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	1281-1285 bp	1.0	76.2
rpoB-trnC-GCA_p3	TTCCCATTTCCATCCCCAAG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1302-1306 bp	1.0	75.6
rpoB-trnC-GCA_p4	GCTCTTCTGTTAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.2	58.1	1382-1386 bp	1.0	73.8
rpoB-trnC-GCA_p5	AGCTCTTCTGTTAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.2	58.1	1383-1387 bp	1.0	73.8