Hotspot

atpH-atpI

LSC · 13604-14325 · 722 bp

Back to Humulus

Pi 0.00284

MarkerSeek score 62.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 2
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	797-809 bp	1.0	80.5
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	853-865 bp	1.0	79.8
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	798-810 bp	1.0	79.4
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.2	57.9	857-869 bp	1.0	79.2
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	812-824 bp	1.0	79.1