Hotspot

trnS-GCU-trnG-UCC

LSC · 8203-9019 · 817 bp

Back to Humulus

Pi 0.00491

MarkerSeek score 60.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 4
pi: 0.00491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ATTCATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	932-935 bp	1.0	77.1
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ATTCATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	931-934 bp	1.0	77.1
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	ATTCATCAAACCGAGGGACC	50.0	50.0	58.2	57.6	986-989 bp	1.0	75.9
trnS-GCU-trnG-UCC_p4	ACGAAAACGGAAAGAGAGGG	ATTCATCAAACCGAGGGACC	50.0	50.0	57.8	57.6	992-995 bp	1.0	75.8
trnS-GCU-trnG-UCC_p5	AGCAATCCGACGCTTTAGTC	ATTCATCAAACCGAGGGACC	50.0	50.0	58.4	57.6	934-937 bp	1.0	74.3