Hotspot

trnK-UUU-rps16

LSC · 4386-5288 · 903 bp

Back to Humulus

Pi 0.00887
MarkerSeek score 57.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
903
variable_sites
8
pi
0.00887
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.6 58.3 1053-1067 bp 1.0 73.6
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG TCAAAGAAGGCTGGGGTTTT 50.0 45.0 58.6 57.5 1008-1022 bp 1.0 72.4
trnK-UUU-rps16_p3 AGTCGCACTTAAAAGCCGAG TTCAAAGAAGGCTGGGGTTT 50.0 45.0 58.6 57.5 1009-1023 bp 1.0 72.4
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG TTTCAAAGAAGGCTGGGGTT 50.0 45.0 58.6 57.5 1010-1024 bp 1.0 72.4
trnK-UUU-rps16_p5 TCGCACTTAAAAGCCGAGTA TTCCTTGAAAAAGGCGCTCA 45.0 45.0 57.3 58.3 1051-1065 bp 1.0 72.3