Hotspot

ycf1

IRa · 145601-151630 · 6030 bp

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Pi 0.00877
MarkerSeek score 55.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRa
length_bp
6030
variable_sites
333
pi
0.00877
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.98
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GCCGAAAGTCCGATTGGTAT CCCGATGGCCAGACAAATTA 50.0 50.0 58.1 57.9 330-495 bp 1.0 91.2
ycf1_p2 TCTGCCCACCCATTTATTGG CCCGATGGCCAGACAAATTA 50.0 50.0 57.8 57.9 865-1030 bp 1.0 88.5
ycf1_p3 GCCGAAAGTCCGATTGGTAT GCAGTGCCAGTCGAATATCA 50.0 50.0 58.1 58.1 265-430 bp 0.925926 88.2
ycf1_p4 CCGCCTTTCTTCCTCGTTTA CCCGATGGCCAGACAAATTA 50.0 50.0 57.9 57.9 605-770 bp 0.925926 86.2
ycf1_p5 CCGCCTTTCTTCCTCGTTTA GCAGTGCCAGTCGAATATCA 50.0 50.0 57.9 58.1 540-705 bp 0.851852 83.4