Hotspot

rpl16

LSC · 87704-89272 · 1569 bp

Back to Hoya

Pi 0.0137
MarkerSeek score 70.80
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1569
variable_sites
168
pi
0.0137
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCACACCCAAGACTCATTTCT TGAATAAAAATCCGATTGACACT 42.9 30.4 56.9 54.6 1653-1928 bp 1.0 52.0
rpl16_p2 ACACCCAAGACTCATTTCTGT TGAATAAAAATCCGATTGACACT 42.9 30.4 57.1 54.6 1651-1926 bp 0.962963 50.5
rpl16_p3 ACACCCAAGACTCATTTCTGT TGAATAAAAATCCGATTGACACTT 42.9 29.2 57.1 55.4 1651-1926 bp 0.962963 50.5
rpl16_p4 ACACCCAAGACTCATTTCTGT TTGAATAAAAATCCGATTGACACT 42.9 29.2 57.1 55.4 1652-1927 bp 0.962963 50.5
rpl16_p5 ACACCCAAGACTCATTTCTGTT TGAATAAAAATCCGATTGACACT 40.9 30.4 57.8 54.6 1651-1926 bp 0.962963 50.5