Hotspot

ndhF

SSC · 132069-134309 · 2241 bp

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Pi 0.0213
MarkerSeek score 66.00
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2241
variable_sites
323
pi
0.0213
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AGAGAAAGAGCGAAGATATGTGA TTCGTTGGATGTGAAAGACA 39.1 40.0 57.2 55.2 2920-3000 bp 0.666667 37.6
ndhF_p2 TTCGTTGGATGTGAAAGACA AGAGAAAGAGCGAAGATATGTGA 40.0 39.1 55.2 57.2 2920-3000 bp 0.666667 37.6
ndhF_p3 CCGAATACGCCTTTTTGATGT TCAAAAAGGTAAGATCCAGTCA 42.9 36.4 57.2 55.0 2765-2806 bp 0.62963 34.0
ndhF_p4 AGAGAAAGAGCGAAGATATGTGA TTTCGTTGGATGTGAAAGACA 39.1 38.1 57.2 56.0 2921-2998 bp 0.592593 33.9
ndhF_p5 CCGAATACGCCTTTTTGATGT CCTTTCAAAAAGGTAAGATCCAGT 42.9 37.5 57.2 57.1 2769-2810 bp 0.481481 26.3