Hotspot

accD

LSC · 63694-65481 · 1788 bp

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Pi 0.0164
MarkerSeek score 65.80
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1788
variable_sites
162
pi
0.0164
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 ACGACATAAGAAAAGGCGCT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.9 58.0 2536-2975 bp 0.925926 87.4
accD_p2 ACGACATAAGAAAAGGCGCT AAGAAGCCATTGCAATTGCC 45.0 45.0 57.9 57.9 2542-2981 bp 0.925926 86.8
accD_p3 ACGACATAAGAAAAGGCGCT TCTAGCGATCCTGGTCGATT 45.0 50.0 57.9 58.0 2789-2979 bp 0.666667 74.1
accD_p4 ACGACATAAGAAAAGGCGCT CTGGTCGATTTGAAAACGGC 45.0 50.0 57.9 58.0 2779-2969 bp 0.666667 74.0
accD_p5 ACGACATAAGAAAAGGCGCT ATCTAGCGATCCTGGTCGAT 45.0 50.0 57.9 57.8 2790-2980 bp 0.62963 70.9