Hotspot

rpl20-rps12

LSC · 75103-75992 · 890 bp

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Pi 0.0173
MarkerSeek score 70.60
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
890
variable_sites
93
pi
0.0173
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACGAGCTATATATCCCCGTCT CTCAACGTCGAGGAACATGT 47.6 50.0 57.4 57.9 905-1010 bp 1.0 77.9
rpl20-rps12_p2 ACGAGCTATATATCCCCGTCT GAGGAACATGTACTCGGGTG 47.6 55.0 57.4 57.7 896-1001 bp 1.0 76.2
rpl20-rps12_p3 TTGTTCTACGTCTACGAGCT CTCAACGTCGAGGAACATGT 45.0 50.0 56.1 57.9 918-1023 bp 1.0 75.5
rpl20-rps12_p4 ACGAGCTATATATCCCCGTCT ACAGCCAATCAGAAATGCCA 47.6 45.0 57.4 58.1 952-1057 bp 0.962963 75.2
rpl20-rps12_p5 ACGAGCTATATATCCCCGTCT CAGCCAATCAGAAATGCCAC 47.6 50.0 57.4 57.7 951-1056 bp 0.962963 73.4