Hotspot

ndhC-trnV-UAC

LSC · 56140-57638 · 1499 bp

Back to Hoya

Pi 0.0258
MarkerSeek score 86.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1499
variable_sites
236
pi
0.0258
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGTTAATCGGGGCT CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1040-1768 bp 1.0 90.1
ndhC-trnV-UAC_p2 TGGTCCTTTGTTAATCGGGG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.5 58.1 1042-1770 bp 1.0 87.1
ndhC-trnV-UAC_p3 GTCCTTTGTTAATCGGGGCT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1025-1753 bp 1.0 85.5
ndhC-trnV-UAC_p4 GGTCCTTTGTTAATCGGGGC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.9 58.1 1041-1769 bp 1.0 83.9
ndhC-trnV-UAC_p5 GTCCTTTGTTAATCGGGGCT CGAGAAAGTCTACGGTTCGA 50.0 50.0 57.9 57.1 1039-1767 bp 1.0 83.9