Hotspot

psaA-ycf3

LSC · 47307-48094 · 788 bp

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Pi 0.0146
MarkerSeek score 72.20
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
788
variable_sites
84
pi
0.0146
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TGAGAAATGACCCGGTTTGG TTGAAGATCACGAGGCGTTT 50.0 45.0 58.1 57.8 914-1008 bp 1.0 88.3
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 895-989 bp 1.0 88.3
psaA-ycf3_p3 TGAGAAATGACCCGGTTTGG TTGGTTGAAGATCACGAGGC 50.0 50.0 58.1 58.2 918-1012 bp 1.0 88.1
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 899-993 bp 1.0 88.0
psaA-ycf3_p5 TGAGAAATGACCCGGTTTGG TGAAGATCACGAGGCGTTTC 50.0 50.0 58.1 58.3 913-1007 bp 1.0 87.3