Hotspot

trnE-UUC-trnT-GGU

LSC · 35948-36970 · 1023 bp

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Pi 0.0262
MarkerSeek score 83.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1023
variable_sites
161
pi
0.0262
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 759-1204 bp 1.0 90.8
trnE-UUC-trnT-GGU_p2 TTTCCTAGTCCCATACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 815-1260 bp 1.0 90.3
trnE-UUC-trnT-GGU_p3 GATGTCCTGAACCGCTAGAC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 758-1203 bp 1.0 90.3
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.9 57.9 724-1169 bp 0.962963 88.5
trnE-UUC-trnT-GGU_p5 AGATGTCCTGAACCGCTAGA ACCGGAGTTTTATGGAAGCC 50.0 50.0 57.9 57.9 792-1237 bp 0.962963 88.4