Hotspot

petN-psbM

LSC · 33770-34575 · 806 bp

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Pi 0.0119
MarkerSeek score 69.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
806
variable_sites
59
pi
0.0119
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCGTAGTGTGGGGAAGAAGT CGTTCTAGTTCCTACCGCCT 50.0 55.0 58.0 58.9 898-1348 bp 1.0 80.7
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCGTTCTAGTTCCTACCGCC 50.0 55.0 58.0 58.9 899-1349 bp 1.0 80.7
petN-psbM_p3 TCGTAGTGTGGGGAAGAAGT TTCGTTCTAGTTCCTACCGC 50.0 50.0 58.0 57.1 900-1350 bp 1.0 80.4
petN-psbM_p4 TTTCCCTTTCGCTCGTAGTG CGTTCTAGTTCCTACCGCCT 50.0 55.0 57.9 58.9 910-1360 bp 1.0 80.2
petN-psbM_p5 TTTCCCTTTCGCTCGTAGTG TCGTTCTAGTTCCTACCGCC 50.0 55.0 57.9 58.9 911-1361 bp 1.0 80.1