Hotspot

rps2-rpoC2

LSC · 16635-21528 · 4894 bp

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Pi 0.0129
MarkerSeek score 59.60
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
4894
variable_sites
410
pi
0.0129
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CTTAGCATACCAAGGGGCTG CTCCGGGGGCTATTAGAGAA 55.0 55.0 58.0 58.0 1151-1293 bp 0.925926 88.7
rps2-rpoC2_p2 CTTAGCATACCAAGGGGCTG TCCGGGGGCTATTAGAGAAG 55.0 55.0 58.0 58.0 1150-1292 bp 0.925926 88.7
rps2-rpoC2_p3 TTATCGTCTGCTTGGGCTTC CGGCATTCTGTTCTACAGCT 50.0 50.0 58.0 58.0 799-955 bp 0.925926 88.7
rps2-rpoC2_p4 TTATCGTCTGCTTGGGCTTC CTCCGGGGGCTATTAGAGAA 50.0 55.0 58.0 58.0 1286-1428 bp 0.888889 87.3
rps2-rpoC2_p5 TTATCGTCTGCTTGGGCTTC TCCGGGGGCTATTAGAGAAG 50.0 55.0 58.0 58.0 1285-1427 bp 0.888889 87.3